Starting with creating our data file data.txt:
12
54
7
332
54
1
0
Here the loop itself has not changed, we've just created a new variable afterwards, mean which is calculated from total and count:
total = 0
count = 0
with open("data.txt") as f:
for line in f:
number = int(line)
total += number
count += 1
mean = total / count
print("Sum of all", count, "values is:", total)
print("The mean is", mean)
python file.py